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Western Blotting

Optimized for success

Sharper images with little to no distortion

Western transfers are an important tool for proteomics research, diagnostics, and forensics applications. This method is widely used to detect the presence of proteins in unknown or complex sample mixtures as well as determining the relative abundance of specific proteins.  Our family of polyvinylidene fluoride (PVDF) membranes have been optimized for compatibility with a wide range of protein stains and enzyme-based detection systems.  The physical characteristics of PVDF membrane, such as durability during stripping and reprobing as well as high tensile strength allowing ease of handling make PVDF an excellent choice for protein detection.

 

 

 

The first step in a western blot is the separation of proteins via gel electrophoresis from a sample containing a mixture of proteins. Once separated on the gel, the proteins are transferred to a (PVDF) membrane or a nitrocellulose membrane. This step immobilizes the proteins for further analysis. The membrane is then incubated with labelled antibodies specific to the protein of interest. After incubation, the membrane is washed to remove any unbound antibodies and further analysed by various immunodetection techniques. 

 

We offer Fluorotrans® PVDF, Fluorotrans® W PVDF, and BioTrace™ NT (nitrocellulose) membranes that have been optimized for western blotting applications. Autofluorescence from standard western transfer membranes can obscure specific signals, especially at lower wavelengths. Our Fluorotrans® PVDF has been optimized for fluorescent detection to ensure a very low background that will not interfere with protein detection and analysis when exposed to fluorescence. Our Fluorotrans® W PVDF is optimal for use with traditional staining and chemiluniscent detection methods exhibiting high sensitivity, low burn-through and low background. The sensitive nature of our membranes yield high resolution with low background and burn through.

 

 

 

Pall’s BioTrace™ NT is a pure nitrocellulose unsupported media that has a high binding capacity for nucleic acids and proteins. This high protein binding capacity is ensured due to the homogenous nature of the membrane. Other nitrocellulose membranes contain high levels of cellulose acetate which reduce the protein binding capacity of the membrane.  Physical characteristics of BioTrace™ NT membrane such as high tensile strength and hydrophilicity provide excellent handling ensuring that the membrane will not rip, tear or crack during transfers. Since the membrane is detergent free and unsupported, it yields sharper images with little to no distortion. Compatible with a variety of detection systems, BioTrace™ NT has lower protein burn through than competitive nitrocellulose media in electrophoretic transfers. Highly consistent, both inter- and intra- lot, this membrane provides sensitive detection of biomolecules blot after blot